cd4 pe vio770 Search Results


560501 cd4 phycoerythrin vio770 pe vio770 miltenyi biotec 130 113 227 cd8 brilliant violet 510  (Miltenyi Biotec)
96
Miltenyi Biotec 560501 cd4 phycoerythrin vio770 pe vio770 miltenyi biotec 130 113 227 cd8 brilliant violet 510
560501 Cd4 Phycoerythrin Vio770 Pe Vio770 Miltenyi Biotec 130 113 227 Cd8 Brilliant Violet 510, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/560501 cd4 phycoerythrin vio770 pe vio770 miltenyi biotec 130 113 227 cd8 brilliant violet 510/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
560501 cd4 phycoerythrin vio770 pe vio770 miltenyi biotec 130 113 227 cd8 brilliant violet 510 - by Bioz Stars, 2026-03
96/100 stars
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cd4 pe vio770  (Miltenyi Biotec)
96
Miltenyi Biotec cd4 pe vio770
( A ) Single-cell sequencing analysis of biopsies from non-small cell lung cancer (NSCLC) patients. Panels indicate the expression of PDCD1 , LAG3 and CBLB and CBLC analyzed from the single-cell lung cancer extended atlas (LuCA) (Salcher et al, ) repository as indicated. ( B ) Dot plot with the percentage of <t>CD4</t> and CD8 T-cells that co-express PD-1 and LAG-3 after ex vivo activation, from healthy donors ( n = 8) and NSCLC patients ( n = 10). Statistical comparisons were performed by the Mann–Whitney test. Error bars correspond to ±SD ( C ) CBL-B expression by mean fluorescent intensities in CD4 and CD8 T-cells from a sample of non-responder NSCLC patients ( n = 4), activated ex vivo in the presence of the indicated treatments. Shown data from total CD4 and CD8 gated populations. Statistical comparisons were carried out by a two-way ANOVA to eliminate inter-patient variability followed by pair-wise Tukey tests. Box and whiskers with min to max values are plotted, computing the minimum, maximum, median and quartiles. The box extends from the 25th to 75th percentiles. The whiskers go down to the smallest value and up to the largest. ( D ) Same as ( C ) but for C-CBL expression. Box and whiskers with min to max values are plotted, computing the minimum, maximum, median and quartiles. The box extends from the 25th to 75th percentiles. The whiskers go down to the smallest value and up to the largest. ( E ) Percentage of proliferating CD4 T cells (left) and CD8 T cells (right) from a sample of high PD-1/LAG-3 co-expression patients before starting immunotherapy, activated ex vivo by A549-SC3 cells in the presence of the indicated antibodies. Statistical comparisons were carried out by a two-way ANOVA to eliminate inter-patient variability followed by pair-wise Tukey tests ( n = 5). Box and whiskers with min to max values are plotted, computing the minimum, maximum, median and quartiles. The box extends from the 25th to 75th percentiles. The whiskers go down to the smallest value and up to the largest. ( F ) Flow cytometry histograms of SATB1, Phospho SMAD 2/3, LCK and ZAP70 expression. Gates were established according to unstained controls in T-cells from a sample of non-responder NSCLC patients. Percentage of expression and Mean Fluorescence Intensity values are indicated. Data information: Statistical comparisons are shown in the graph as indicated in Methods. Briefly, for ( B ) statistical comparisons were performed by the Mann–Whitney test. For ( C – E ), statistical comparisons were carried out by a two-way ANOVA to eliminate inter-patient variability followed by pair-wise Tukey tests. Error bars correspond to ±SD. **, ***, ****, indicate P < 0.01, P < 0.001 and P < 0.0001 differences. .
Cd4 Pe Vio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd4 pe vio770/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
cd4 pe vio770 - by Bioz Stars, 2026-03
96/100 stars
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anti human cd4 pe vio770  (Miltenyi Biotec)
96
Miltenyi Biotec anti human cd4 pe vio770
( A ) Single-cell sequencing analysis of biopsies from non-small cell lung cancer (NSCLC) patients. Panels indicate the expression of PDCD1 , LAG3 and CBLB and CBLC analyzed from the single-cell lung cancer extended atlas (LuCA) (Salcher et al, ) repository as indicated. ( B ) Dot plot with the percentage of <t>CD4</t> and CD8 T-cells that co-express PD-1 and LAG-3 after ex vivo activation, from healthy donors ( n = 8) and NSCLC patients ( n = 10). Statistical comparisons were performed by the Mann–Whitney test. Error bars correspond to ±SD ( C ) CBL-B expression by mean fluorescent intensities in CD4 and CD8 T-cells from a sample of non-responder NSCLC patients ( n = 4), activated ex vivo in the presence of the indicated treatments. Shown data from total CD4 and CD8 gated populations. Statistical comparisons were carried out by a two-way ANOVA to eliminate inter-patient variability followed by pair-wise Tukey tests. Box and whiskers with min to max values are plotted, computing the minimum, maximum, median and quartiles. The box extends from the 25th to 75th percentiles. The whiskers go down to the smallest value and up to the largest. ( D ) Same as ( C ) but for C-CBL expression. Box and whiskers with min to max values are plotted, computing the minimum, maximum, median and quartiles. The box extends from the 25th to 75th percentiles. The whiskers go down to the smallest value and up to the largest. ( E ) Percentage of proliferating CD4 T cells (left) and CD8 T cells (right) from a sample of high PD-1/LAG-3 co-expression patients before starting immunotherapy, activated ex vivo by A549-SC3 cells in the presence of the indicated antibodies. Statistical comparisons were carried out by a two-way ANOVA to eliminate inter-patient variability followed by pair-wise Tukey tests ( n = 5). Box and whiskers with min to max values are plotted, computing the minimum, maximum, median and quartiles. The box extends from the 25th to 75th percentiles. The whiskers go down to the smallest value and up to the largest. ( F ) Flow cytometry histograms of SATB1, Phospho SMAD 2/3, LCK and ZAP70 expression. Gates were established according to unstained controls in T-cells from a sample of non-responder NSCLC patients. Percentage of expression and Mean Fluorescence Intensity values are indicated. Data information: Statistical comparisons are shown in the graph as indicated in Methods. Briefly, for ( B ) statistical comparisons were performed by the Mann–Whitney test. For ( C – E ), statistical comparisons were carried out by a two-way ANOVA to eliminate inter-patient variability followed by pair-wise Tukey tests. Error bars correspond to ±SD. **, ***, ****, indicate P < 0.01, P < 0.001 and P < 0.0001 differences. .
Anti Human Cd4 Pe Vio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti human cd4 pe vio770/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
anti human cd4 pe vio770 - by Bioz Stars, 2026-03
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cd4  (Miltenyi Biotec)
94
Miltenyi Biotec cd4
List containing all antibodies utilized for surface staining of mass cytometry samples.
Cd4, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd4/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
cd4 - by Bioz Stars, 2026-03
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phycoerythrin pe vio770  (Miltenyi Biotec)
93
Miltenyi Biotec phycoerythrin pe vio770
List containing all antibodies utilized for surface staining of mass cytometry samples.
Phycoerythrin Pe Vio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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cd4 antibody, anti-mouse, reafinity  (Miltenyi Biotec)
95
Miltenyi Biotec cd4 antibody, anti-mouse, reafinity
List containing all antibodies utilized for surface staining of mass cytometry samples.
Cd4 Antibody, Anti Mouse, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd4 antibody, anti-mouse, reafinity/product/Miltenyi Biotec
Average 95 stars, based on 1 article reviews
cd4 antibody, anti-mouse, reafinity - by Bioz Stars, 2026-03
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Image Search Results


( A ) Single-cell sequencing analysis of biopsies from non-small cell lung cancer (NSCLC) patients. Panels indicate the expression of PDCD1 , LAG3 and CBLB and CBLC analyzed from the single-cell lung cancer extended atlas (LuCA) (Salcher et al, ) repository as indicated. ( B ) Dot plot with the percentage of CD4 and CD8 T-cells that co-express PD-1 and LAG-3 after ex vivo activation, from healthy donors ( n = 8) and NSCLC patients ( n = 10). Statistical comparisons were performed by the Mann–Whitney test. Error bars correspond to ±SD ( C ) CBL-B expression by mean fluorescent intensities in CD4 and CD8 T-cells from a sample of non-responder NSCLC patients ( n = 4), activated ex vivo in the presence of the indicated treatments. Shown data from total CD4 and CD8 gated populations. Statistical comparisons were carried out by a two-way ANOVA to eliminate inter-patient variability followed by pair-wise Tukey tests. Box and whiskers with min to max values are plotted, computing the minimum, maximum, median and quartiles. The box extends from the 25th to 75th percentiles. The whiskers go down to the smallest value and up to the largest. ( D ) Same as ( C ) but for C-CBL expression. Box and whiskers with min to max values are plotted, computing the minimum, maximum, median and quartiles. The box extends from the 25th to 75th percentiles. The whiskers go down to the smallest value and up to the largest. ( E ) Percentage of proliferating CD4 T cells (left) and CD8 T cells (right) from a sample of high PD-1/LAG-3 co-expression patients before starting immunotherapy, activated ex vivo by A549-SC3 cells in the presence of the indicated antibodies. Statistical comparisons were carried out by a two-way ANOVA to eliminate inter-patient variability followed by pair-wise Tukey tests ( n = 5). Box and whiskers with min to max values are plotted, computing the minimum, maximum, median and quartiles. The box extends from the 25th to 75th percentiles. The whiskers go down to the smallest value and up to the largest. ( F ) Flow cytometry histograms of SATB1, Phospho SMAD 2/3, LCK and ZAP70 expression. Gates were established according to unstained controls in T-cells from a sample of non-responder NSCLC patients. Percentage of expression and Mean Fluorescence Intensity values are indicated. Data information: Statistical comparisons are shown in the graph as indicated in Methods. Briefly, for ( B ) statistical comparisons were performed by the Mann–Whitney test. For ( C – E ), statistical comparisons were carried out by a two-way ANOVA to eliminate inter-patient variability followed by pair-wise Tukey tests. Error bars correspond to ±SD. **, ***, ****, indicate P < 0.01, P < 0.001 and P < 0.0001 differences. .

Journal: EMBO Molecular Medicine

Article Title: PD-1/LAG-3 co-signaling profiling uncovers CBL ubiquitin ligases as key immunotherapy targets

doi: 10.1038/s44321-024-00098-y

Figure Lengend Snippet: ( A ) Single-cell sequencing analysis of biopsies from non-small cell lung cancer (NSCLC) patients. Panels indicate the expression of PDCD1 , LAG3 and CBLB and CBLC analyzed from the single-cell lung cancer extended atlas (LuCA) (Salcher et al, ) repository as indicated. ( B ) Dot plot with the percentage of CD4 and CD8 T-cells that co-express PD-1 and LAG-3 after ex vivo activation, from healthy donors ( n = 8) and NSCLC patients ( n = 10). Statistical comparisons were performed by the Mann–Whitney test. Error bars correspond to ±SD ( C ) CBL-B expression by mean fluorescent intensities in CD4 and CD8 T-cells from a sample of non-responder NSCLC patients ( n = 4), activated ex vivo in the presence of the indicated treatments. Shown data from total CD4 and CD8 gated populations. Statistical comparisons were carried out by a two-way ANOVA to eliminate inter-patient variability followed by pair-wise Tukey tests. Box and whiskers with min to max values are plotted, computing the minimum, maximum, median and quartiles. The box extends from the 25th to 75th percentiles. The whiskers go down to the smallest value and up to the largest. ( D ) Same as ( C ) but for C-CBL expression. Box and whiskers with min to max values are plotted, computing the minimum, maximum, median and quartiles. The box extends from the 25th to 75th percentiles. The whiskers go down to the smallest value and up to the largest. ( E ) Percentage of proliferating CD4 T cells (left) and CD8 T cells (right) from a sample of high PD-1/LAG-3 co-expression patients before starting immunotherapy, activated ex vivo by A549-SC3 cells in the presence of the indicated antibodies. Statistical comparisons were carried out by a two-way ANOVA to eliminate inter-patient variability followed by pair-wise Tukey tests ( n = 5). Box and whiskers with min to max values are plotted, computing the minimum, maximum, median and quartiles. The box extends from the 25th to 75th percentiles. The whiskers go down to the smallest value and up to the largest. ( F ) Flow cytometry histograms of SATB1, Phospho SMAD 2/3, LCK and ZAP70 expression. Gates were established according to unstained controls in T-cells from a sample of non-responder NSCLC patients. Percentage of expression and Mean Fluorescence Intensity values are indicated. Data information: Statistical comparisons are shown in the graph as indicated in Methods. Briefly, for ( B ) statistical comparisons were performed by the Mann–Whitney test. For ( C – E ), statistical comparisons were carried out by a two-way ANOVA to eliminate inter-patient variability followed by pair-wise Tukey tests. Error bars correspond to ±SD. **, ***, ****, indicate P < 0.01, P < 0.001 and P < 0.0001 differences. .

Article Snippet: The following antibodies were used at 1:50 dilution unless otherwise stated: CD4-APC-Vio770 (clone M-T466, Miltenyi), CD3-APC (clone REA613, Milenyi Biotec), CD28-PECy7 (clone CD28.2, Biolegend), PD-1-PE (clone EH12.2H7, Biolegend), CD8-FITC (clone SDK1, Biolegend), LAG3-PE (clone 11C3C65, Biolegend), LAG3-PerCP-Cy5.5 (clone 11C3C65, Biolegend), CD4-FITC (clone REA623, Milteny), CD3-PerCP-Cy5.5 (clone T100, TONBO), CD4-PE-Vio770 (clone REA261, Milteny), CD223-PerCP-Cy5 (clone 11C3C65, Biolegend), PD-1-Pacific Blue (clone EH12.2H7, Biolegend).

Techniques: Sequencing, Expressing, Ex Vivo, Activation Assay, MANN-WHITNEY, Flow Cytometry, Fluorescence

( A ) Schematic design of the experiment. BALB/c female mice were randomly allocated and subcutaneously injected with 2 × 10 6 Lung adenocarcinoma (Lacun3) cells per animal. 100 µg of anti-PD-1, 100 µg of anti-LAG3, 30 mg/kg of CBL-Bi and the corresponding depletion antibodies were administered intraperitoneally at days 0, 2, 6, 9, 13 and 15 as indicated in the figure. NK, CD4, and CD8 T‐cell depletions were carried out by intraperitoneal administration of 100 μg of anti‐mouse CD8a, CD4 or NK1.1 antibody. Mice were humanely sacrificed when tumor size reached ~150–200 mm 2 , or when tumor ulceration or discomfort were observed. ( B ) Kaplan–Meier survival plot of mice under the indicated treatments or depletion (percent). Statistical significance was tested with the Log-rank test. ( C ) Evolution of mean tumor size following the indicated treatments (left). Tumor volumes 9 days after treatment initiation (right). Error bars correspond to ±SEM (left) and box and whiskers with min to max values (right), computing the minimum, maximum, median and quartiles for 25th and 75th percentiles. The whiskers go down to the smallest value and up to the largest ( n = 6 mice per group). Data information: Statistical comparisons were carried out by a two-way ANOVA followed by pair-wise Tukey tests. ( D ) Tumor growth of individual mice in the indicated treatment groups ( n = 6 mice per group). Statistical comparisons are shown in the graph as indicated in Methods. Data information: Briefly, for ( B ), survival was represented by Kaplan–Meier plots and analyzed by log-rank test. For ( C ), statistical comparisons were carried out by a two-way ANOVA followed by pair-wise Tukey tests. *, **, ****, indicate P < 0.05, P < 0.01, and P < 0.0001 differences. .

Journal: EMBO Molecular Medicine

Article Title: PD-1/LAG-3 co-signaling profiling uncovers CBL ubiquitin ligases as key immunotherapy targets

doi: 10.1038/s44321-024-00098-y

Figure Lengend Snippet: ( A ) Schematic design of the experiment. BALB/c female mice were randomly allocated and subcutaneously injected with 2 × 10 6 Lung adenocarcinoma (Lacun3) cells per animal. 100 µg of anti-PD-1, 100 µg of anti-LAG3, 30 mg/kg of CBL-Bi and the corresponding depletion antibodies were administered intraperitoneally at days 0, 2, 6, 9, 13 and 15 as indicated in the figure. NK, CD4, and CD8 T‐cell depletions were carried out by intraperitoneal administration of 100 μg of anti‐mouse CD8a, CD4 or NK1.1 antibody. Mice were humanely sacrificed when tumor size reached ~150–200 mm 2 , or when tumor ulceration or discomfort were observed. ( B ) Kaplan–Meier survival plot of mice under the indicated treatments or depletion (percent). Statistical significance was tested with the Log-rank test. ( C ) Evolution of mean tumor size following the indicated treatments (left). Tumor volumes 9 days after treatment initiation (right). Error bars correspond to ±SEM (left) and box and whiskers with min to max values (right), computing the minimum, maximum, median and quartiles for 25th and 75th percentiles. The whiskers go down to the smallest value and up to the largest ( n = 6 mice per group). Data information: Statistical comparisons were carried out by a two-way ANOVA followed by pair-wise Tukey tests. ( D ) Tumor growth of individual mice in the indicated treatment groups ( n = 6 mice per group). Statistical comparisons are shown in the graph as indicated in Methods. Data information: Briefly, for ( B ), survival was represented by Kaplan–Meier plots and analyzed by log-rank test. For ( C ), statistical comparisons were carried out by a two-way ANOVA followed by pair-wise Tukey tests. *, **, ****, indicate P < 0.05, P < 0.01, and P < 0.0001 differences. .

Article Snippet: The following antibodies were used at 1:50 dilution unless otherwise stated: CD4-APC-Vio770 (clone M-T466, Miltenyi), CD3-APC (clone REA613, Milenyi Biotec), CD28-PECy7 (clone CD28.2, Biolegend), PD-1-PE (clone EH12.2H7, Biolegend), CD8-FITC (clone SDK1, Biolegend), LAG3-PE (clone 11C3C65, Biolegend), LAG3-PerCP-Cy5.5 (clone 11C3C65, Biolegend), CD4-FITC (clone REA623, Milteny), CD3-PerCP-Cy5.5 (clone T100, TONBO), CD4-PE-Vio770 (clone REA261, Milteny), CD223-PerCP-Cy5 (clone 11C3C65, Biolegend), PD-1-Pacific Blue (clone EH12.2H7, Biolegend).

Techniques: Injection

List containing all antibodies utilized for surface staining of mass cytometry samples.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: List containing all antibodies utilized for surface staining of mass cytometry samples.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Staining, Mass Cytometry, Marker

General gating strategy for identified immune cell subsets and the used marker combination for definition.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: General gating strategy for identified immune cell subsets and the used marker combination for definition.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Marker

Influence of diet-induced obesity on circulating murine immune cells. Uniform manifold approximation and projection (UMAP) with clusters from FlowSOM analysis (Ly6G, CD115, CD4, CD11b, CD19, CD3e, TCRgd, Ly6C, NKp46, CD8a, NK1.1, B220, and CD11c) in mice receiving either low-fat diet (LFD) or high-fat diet (HFD) (A) . Z -score normalized heatmap indicating percentages of immune cell populations in lean and obese mice (B) as well as their actual percentages (C) . Statistics: unpaired two-tailed Student’s t -test to compare the populations between lean and obese mice. ▪ indicates p ≤ 0.1, * indicates p ≤ 0.05, ** indicates p ≤ 0.01. Sample sizes: LFD CTRL = 5, HFD CTRL = 5. Data are displayed as mean ± SEM. ns, not significant.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: Influence of diet-induced obesity on circulating murine immune cells. Uniform manifold approximation and projection (UMAP) with clusters from FlowSOM analysis (Ly6G, CD115, CD4, CD11b, CD19, CD3e, TCRgd, Ly6C, NKp46, CD8a, NK1.1, B220, and CD11c) in mice receiving either low-fat diet (LFD) or high-fat diet (HFD) (A) . Z -score normalized heatmap indicating percentages of immune cell populations in lean and obese mice (B) as well as their actual percentages (C) . Statistics: unpaired two-tailed Student’s t -test to compare the populations between lean and obese mice. ▪ indicates p ≤ 0.1, * indicates p ≤ 0.05, ** indicates p ≤ 0.01. Sample sizes: LFD CTRL = 5, HFD CTRL = 5. Data are displayed as mean ± SEM. ns, not significant.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Two Tailed Test

Analysis of essential components of signal transduction of the inflammatory reflex in the spleen. Occurrence of ChAT + CD4 + T cells in the spleen of lean and obese mice (A) . Statistics: Comparison of lean and obese mice was achieved by an unpaired two-tailed Student’s t -test. Comparison of TNF-α + splenic macrophages after LPS stimulation with or without different concentrations of nicotine treatment to the control (B) were analyzed using a two-way ANOVA with repeated measurements followed by an uncorrected Fisher’s LSD test. * indicates p ≤ 0.05. Sample sizes: Each group at for each analysis and condition: n = 5. Data are displayed as mean ± SEM.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: Analysis of essential components of signal transduction of the inflammatory reflex in the spleen. Occurrence of ChAT + CD4 + T cells in the spleen of lean and obese mice (A) . Statistics: Comparison of lean and obese mice was achieved by an unpaired two-tailed Student’s t -test. Comparison of TNF-α + splenic macrophages after LPS stimulation with or without different concentrations of nicotine treatment to the control (B) were analyzed using a two-way ANOVA with repeated measurements followed by an uncorrected Fisher’s LSD test. * indicates p ≤ 0.05. Sample sizes: Each group at for each analysis and condition: n = 5. Data are displayed as mean ± SEM.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Transduction, Comparison, Two Tailed Test, Control